RE:GFP antibodies

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From:Tamara Howard <>
To:Histology listserver <>
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On the use of antibodies to detect GFP....A lot depends on your construct
- what you have the FP hooked up with. I've used several of the anti-FP
antibodies, from several different vendors, and had some success. But with the
same fixation, same cells/tissue, same GFP vector, same antibody, some
constructs will label and others won't. I know this is a pain, but the
best advice I can give is to try the antibody on your sample and see what

Oh, and anyone who tells you, "But it works on Westerns",  should be
taken out and shot :)

Good luck!

Tamara Howard

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