Slide Adhesive for Bone

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From:Jay Turner <>
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I've been having a lot of trouble with sections lifting during the heating 
steps of anitgen retrieval for IHC stains on bone samples.  There isn't any 
problem with the surrounding soft tissue (i.e. muscle), but the cortical 
bone refuses to stay on the slide.  I've tried using Plus slides, coating 
with poly-L-lysine, casein glue...almost everything that's out there.

Does anyone have any suggestions for either an alternative slide adhesive or 
an alternative step that would be just as effective as HIER in bone?

Thanks in advance.


Jay D. Turner
Dept. of Orthopaedics
University of Medicine and Dentistry of New Jersey
185 South Orange Ave.
Newark, NJ 07103
Phone: 973-972-4408
Fax: 973-972-5294

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