Re: p27 and Cyclin E IHC

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From:Luis Chiriboga <luis.chiriboga@med.nyu.edu>
To:"Trivett Melanie (PMC)" <TrivettMelanie@petermac.unimelb.edu.au>, Histonet <Histonet@pathology.swmed.edu>
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Hi
Use Nova Castra Cyclin E (13a3) mouse anti-human, HEIR Emerson model MW8106W,
1000 Watt  microwave 20minute in 0.01M Citrate pH6.0,  30 minute cool,  1:20
overnight incubation, Ventana DAB Detection (nexus). Good S/N.

Use  Santa Cruz p27 (F8) mouse anti-human, HEIR Emerson model MW8106W, 1000
Watt  microwave 20 minute in 0.01M Citrate pH6.0,  30 minute cool,  1:10
overnight incubation, Ventana DAB Detection (nexus). Poor S/N.

Hope this helps
regards luis



"Trivett Melanie (PMC)" wrote:

> Hello All,
>
> Is anyone out there doing immunohistochemistry on FFPE sections for the
> above antigens? (Human tissue).  I am looking for sources/recommendations
> for antibodies.
>
> Thanks in advance for any help.
>
> Melanie.
>
> Melanie Trivett, PhD.
> Immunohistochemistry Laboratory,
> Department of Pathology,
> Peter MacCallum Cancer Institute.
>
> Tel: 03 9656 1431
> Fax: 03 9656 1460
> Email: TrivettMelanie@petermac.unimelb.edu.au

--
Luis Chiriboga Ph.D HT(ASCP)QIHC
Department of Pathology 4W27
Bellevue Hospital
(212)562-4667






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