I have found some antigens respond well to pressure cooking and microwave, B5 
fixed tissue does not, other clones like enzyme and hate steam or pressure or 
microwave, I have been doing all three or should I say 4 if we include 
enzymes for about 5 yrs and it is all trial and error, clone dependent, 
fixation driven, dtection chemistry sensitive. So if you enjoy , discovery, 
playing with antibodies, and trial and error , have fun. Please be aware that 
you can over retrieve as well and force false positivity, I did it with some 
liver tissue and a clone in test cases , confirmed by pcr.
This is a great field.                          Dana