Date sent:      	Mon, 08 May 2000 14:05:28 -0700
From:           	Carrie Cartwright <Carrie.Cartwright@Agouron.COM>
Subject:        	Davidson's fixative
To:             	"histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu>

> Can anyone give me the protocol for making Davidson's fixative and nay
> information you may have on it?

Carrie,
This is the formula I have for Davidsons fixative. We use it as a 
post formalin fix for mouse eyes to improve penetration and initiate 
dehydration (12-24 hours) then into 50% alcohol.

95% IMS  - 30ml
10% neutral formalin - 20ml
glacial acetic acid - 10ml
distilled water - 30ml