Hi,
    No I don't mind at all. In fact I will even offer our answer to the CD5. We
use DAKO epitope retrieval solution in a steamer for 20 min. H2O2 for 5 min,
then CD5 (also from DAKO) diluted to 1:400 for 30 min. The detection kit we use
for this one is Envision+ for mouse for 30 min. All at room temp. We get very
good results with this.
Good luck
Amos Brooks

Marylou Pohl wrote:

> If you don't mind me piggy-backing on this message, along with CD10, CD5 is
> also a hematopathology stain to contend with.  Same questions apply.
>
> Marylou Pohl
>
> Amos Brooks wrote:
>
> > Hey All,
> >     One of our hematopathologists asked for an an antibody to be worked
> > up that we dont have yet. Does anyone have any suggestions for CD10 on
> > formalin fixed paraffin embedded sections. Please include which
> > detection kit you use and any pretreatments and titrations you use.
> > Thanks much,
> > Amos Brooks