Shot multiple times, then drawn and quartered.
Brett

> ----------
> From: 	Tamara Howard[SMTP:howard@cshl.org]
> Sent: 	Thursday, May 11, 2000 9:22 AM
> To: 	Histology listserver
> Subject: 	RE:GFP antibodies
> 
> On the use of antibodies to detect GFP....A lot depends on your construct
> - what you have the FP hooked up with. I've used several of the anti-FP
> antibodies, from several different vendors, and had some success. But with
> the
> same fixation, same cells/tissue, same GFP vector, same antibody, some
> constructs will label and others won't. I know this is a pain, but the
> best advice I can give is to try the antibody on your sample and see what
> happens! 
> 
> Oh, and anyone who tells you, "But it works on Westerns",  should be
> taken out and shot :)
> 
> Good luck!
> 
> Tamara Howard
> CSHL
> 
> 
>