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We fix our large animal eyes in 5% glutaraldehyde 18-24 hours.
<br>Wash in running water 3-4 hours ,place in 10% NBF for at least 3 days.
<br>Day 1:
<br>Wash eyes 3-4 hours
<br>50% EtOH 2-3 hours
<br>70% EtOH overnight.
<br>Day 2:
<br> Place eyes in 80% EtOH, cut callottes after 7 hours ans
place eyes in 90% overnight.
<br>Day 3:
<br>Two changes of 95%.
<br>Day 4:
<br><tt>Three changes of 100% EtOH and then process.</tt>
<p><tt>It takes quite awhile but anyone who looks at our eyes says they
are amazing!</tt>
<p><tt>Susan Wells</tt>
<br>
<br>
<p>Sharon E Willman wrote:
<blockquote TYPE=CITE>Hi,
<br>I was wondering what your methods are for glutaraldehye fixed
<br>canine and monkey eyes. We find that the eyes are soft when
<br>trying to trim. We put windows in the day of necropsy and
<br>approximately 24 hours later we trim the eyes. Does anyone have
<br>any suggestions as to their procedure on fixation of eyes and
<br>trimming.
<p>Thanks</blockquote>
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