Hi Marylou,

We use Novocastra's (Vector Labs) CD5 at 1:15 on Ventana immunostainers.
Our HIER procedure is 40" 1mM EDTA, pH 8 in a 93 degree C waterbath or the
same buffer for 3" in the Biocare Decloaking Chamber.  As with CD10, we
amplify the primary signal with Ventana's Amplification kit or you can
manually do this by applying RxM IgG followed by MxR IgG after the primary.
Your secondary needs to recognize both mouse and rabbit.  Incubation of the
primary (and all other reagents) is performed at 42 degrees C.  

Hope this helps.

P.S. Access to a good mantle cell lymphoma or CLL is critical for
optimization.

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI  53792-2472


(608)265-6596
FAX: (608)263-1568

> -----Original Message-----
> From:	Marylou Pohl [SMTP:mbpohl@acsu.buffalo.edu]
> Sent:	Sunday, April 30, 2000 1:19 PM
> To:	Amos Brooks
> Cc:	histonet
> Subject:	Re: CD10 ???
> 
> If you don't mind me piggy-backing on this message, along with CD10, CD5
> is
> also a hematopathology stain to contend with.  Same questions apply.
> 
> Marylou Pohl
> 
> Amos Brooks wrote:
> 
> > Hey All,
> >     One of our hematopathologists asked for an an antibody to be worked
> > up that we dont have yet. Does anyone have any suggestions for CD10 on
> > formalin fixed paraffin embedded sections. Please include which
> > detection kit you use and any pretreatments and titrations you use.
> > Thanks much,
> > Amos Brooks << File: Card for Marylou Pohl >>