RE: CD10 ???

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From:"Sebree Linda A." <>
To:"'Amos Brooks'" <>

Hi Amos,

We use Novocastra's (Vector Labs) CD10, clone 56C6 at 1:10.  Our staining
procedure is on a Ventana immunostainer with their DAB detection system.  We
pretreat with HIER:  20" 1mM EDTA, pH 8 in a 93 degree waterbath or 2" EDTA
in the Biocare Decloaking Chamber.  We amplify the signal utilizing
Ventana's Amplification kit after the primary; this can be simulated
manually by applying RxM IgG followed by MxR IgG.  Your secondary would need
to recognize both rabbit and mouse.  

Hope this is of some benefit.

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
600 Highland Avenue
Madison, WI  53792-2472

FAX: (608)263-1568

> -----Original Message-----
> From:	Amos Brooks []
> Sent:	Saturday, April 29, 2000 1:47 PM
> To:	histonet
> Subject:	CD10 ???
> Hey All,
>     One of our hematopathologists asked for an an antibody to be worked
> up that we dont have yet. Does anyone have any suggestions for CD10 on
> formalin fixed paraffin embedded sections. Please include which
> detection kit you use and any pretreatments and titrations you use.
> Thanks much,
> Amos Brooks

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