I have to agree with Andi. coverglass thickness makes a big difference at
the research level. We have to take photos that are publishable quality.
Index of refraction etc. makes a big difference. Photos are not as forgiving
as your eyes.  I believe everything in the light path should be taken into
consideration including sample, coverglass, mountant and oil/air. The optics
in most scopes (please someone correct me if I am mistaken) are set for 1.5
standard coverglass especially for confocal microscopy. AJ











.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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