processing mouse arteries

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From:alexis <>
Date:Wed, 24 Mar 1999 14:18:57 -0500
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Dear histonetters,
I have been processing mouse carotid arteries placed in Histo screen biopsy casettes (Shandon) after fixation in 1% paraformaldehyde (+-1hr.). I process them in a Shandon Citadel 2000 for 30 min. in each bath (starting 70% etoh , 80%, 95%X2, 100% x2, Histoclear (Amresco) x2and paraffin (vacuum) x2. I am consistently getting "beef jerky" impossible to section tissue, any ideas on what I'm doing wrong?  I will try any suggestions!!!

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