Bonnie,

I asked a similar question on Histonet a few months ago.  From the
suggestions I recieved and the subsequent experience I found staining for
Collagen III this was my conclusion.

Pretreat the sections with citrate buffer in a microwave oven for antigen
retrieval for 10 minutes (with our procedure this means the solution is
only boiling for about 1 minute) followed by incubation for 30 mins in
prediluted super sensitive monoclonal antibody purchased from BioGenex.  A
triple stage immunoperoxidase procedure was used.  Excellent staining.
BioGenex recommend pretreated with pepsin, but I found this not
satisfactory.

Hope this may be of help

Neil Hand,
Queen's Medical Centre,
University Hospital,
Nottingham,
England UK.






Bonnie Whitaker wrote,

I'm in need of Type III Collagen that works on formalin-fixed,
paraffin-embedded tissue.  I sure hope that someone out there has some
experience to share on this subject.  Thanks in advance!

UT--Houston Medical School

____________________________________________________________________
Neil Hand
Department Histopathology, University Hospital, Nottingham NG7 2UH.
work : Tel: (0115) 924 9924 extension 43725
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