First of all if the tissues are not trimmed the way the pathologist
should have , they will not process properly, even if they have been
fixed for a week. The process time sounds ok make sure the solutions are
at least half fresh. Then you should not have a problem, let me know,
and Good Luck


Tami Lee wrote:
> 
> Hi everyone,
>         I am trying to fix, process, and section breast and uterine tissue.
> Although the uterus was fixed
> in 10% NBF for several days, it still appeared to have areas that were poorly
> fixed.  The processing
> schedules that I have been using are 12 hours long, and do not seem to be
> effective, especially
> on the breast tissue.  Does anyone have any advice they wouldn't mind sharing?
> I have not been able
> to find anything in our laboratory's textbooks.
> Thanks in advance,
> Tami Lee