Bernice,
	I would be interested to know of specific antigens that are lost in
the heat of the water bath. Can you provide a reference?
Thank-you

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	Instrumedics, Inc.[SMTP:info@instrumedics.com]
> Sent: 	Thursday, March 11, 1999 7:57 AM
> To: 	HistoNet Server
> Subject: 	sticky slide 2
> 
> I wanted to amplify my message of 3/10. The tape-transfer method for
> paraffin sections also uses an adhesive-coated slide which firmly bonds
> the
> section--no loss with any protocol no matter how rigorous!
> 
> The section is supported and captured on a tape window as it is cut  and
> is
> then transferred to the slide. The adhesive coating is polymerized in
> 30-60
> sec. and the section is ready for deparaffinization and staining. The
> water
> bath and drying steps are eliminated. Eliminating the water bath can be
> important in some circumstances; antigenic activity of some epitopes can
> be
> lost in the heated water bath!
> 
> This method has also become valuable for  new research using micro-arrays
> of
> tissues. For  details see the "PSA" product on our web site
> www.instrumedics.com.
> 
> Bernice
> schiller@instrumedics.com
> 
>