Hello,
InnoGenex has two monoclonal anti-Actins: Skeletal Muscle, Smooth 
muscle:both are well validated for human tissue. The anti-Actin polyclonal 
(rabbit)  is validated for human and mouse tissue:This pAb stains I-Bands 
in skeletal muscle.
Additionally, you shouldn't feel constrained to using non-mouse primary 
antibodies when staining mouse tissue--The ISO-IHC Kit that enables 
'mouse-on-mouse' IHC and produces signal:noise ratios that are better than 
most human-tissue stains (no background staining).

M.Ogdie


----------
From: 	rschoonh@sph.unc.edu[SMTP:rschoonh@sph.unc.edu]
Sent: 	Thursday, March 11, 1999 8:14 AM
To: 	Ian Montgomery; HistoNet@pathology.swmed.edu
Subject: 	Re: Smooth muscle.

Ian,

We use Dako's antibody for smooth muscle actin.  It works quite nicely on
human and rat, I would assume that it will work on mice but we haven't
tryed that yet.  We use a 1:100 dilution of the primary 10 min. at room
temp. and we use Dako's Envision kit secondary is also 10 min. at RT.
Works like a charm and it's quick and simple.

-- Begin original message --

>
>
>       Anyone using an good reliable antibody for smooth muscle cells.
> Will be staining human and possibly mouse tissue.
> Ian.
>
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk

-- End original message --

best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**Suppose you were an idiot... And suppose you were a member of Congress
...
But I repeat myself.-Mark Twain**