RE: Smooth muscle.

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From:Matthew Ogdie <>
To:"''" <>
Date:Thu, 11 Mar 1999 09:12:48 -0800
Content-Type:text/plain; charset="us-ascii"

InnoGenex has two monoclonal anti-Actins: Skeletal Muscle, Smooth 
muscle:both are well validated for human tissue. The anti-Actin polyclonal 
(rabbit)  is validated for human and mouse tissue:This pAb stains I-Bands 
in skeletal muscle.
Additionally, you shouldn't feel constrained to using non-mouse primary 
antibodies when staining mouse tissue--The ISO-IHC Kit that enables 
'mouse-on-mouse' IHC and produces signal:noise ratios that are better than 
most human-tissue stains (no background staining).


Sent: 	Thursday, March 11, 1999 8:14 AM
To: 	Ian Montgomery;
Subject: 	Re: Smooth muscle.


We use Dako's antibody for smooth muscle actin.  It works quite nicely on
human and rat, I would assume that it will work on mice but we haven't
tryed that yet.  We use a 1:100 dilution of the primary 10 min. at room
temp. and we use Dako's Envision kit secondary is also 10 min. at RT.
Works like a charm and it's quick and simple.

-- Begin original message --

>       Anyone using an good reliable antibody for smooth muscle cells.
> Will be staining human and possibly mouse tissue.
> Ian.
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail:

-- End original message --

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123

**Suppose you were an idiot... And suppose you were a member of Congress
But I repeat myself.-Mark Twain**

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