Dear Ian,
Anti -alpha smooth muscle actin clone 1A4 works on human and rat tissue
so it should also work on mouse.  I must admit I can't remember if I
have tried it on mouse or not.  It is available from many suppliers but
I have found that Sigma (A-2547) is a good source since it has a much
higher titre than most.  They recommend 1:400 but I have found it works
well at 1:800.  If working with animal tissue the high titre can be an
advantage since dilutions can be radically different.

Penny Marr
> -----Original Message-----
> From:	Ian Montgomery [SMTP:ian.montgomery@bio.gla.ac.uk]
> Sent:	Friday, 12 March 1999 1:32
> To:	HistoNet@pathology.swmed.edu
> Subject:	Smooth muscle.
> 
> 	Anyone using an good reliable antibody for smooth muscle cells.
> Will be staining human and possibly mouse tissue.
> Ian.
> 
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ,
> Scotland.
> Tel: 0141 339 8855 Extn. 6602.
> Fax: 0141 330 4100.
> e-mail: ian.montgomery@bio.gla.ac.uk
> 
> 
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