RE: Formalin/Alcohol Question

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From:Tony Henwood <henwood@mail.one.net.au> (by way of histonet)
To:histonet <histonet@magicnet.net>
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> John,
> our experience with formalin substitutes such as alcoholic formalin and zenk
> formalin have not been good.  We have a tendency to get increased
> non-specific background staining.
>
Have you tried to further dilute your localising antibodies. Perhaps
the antigens are better preserved after the different fixatives.
Tony Henwood
www2.one.net.au/~henwood




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