A 0.5% solution of basic fuchsin or crystal violet should do it. These wouldn't work on cells with a lot of cytoplasm because the cytoplasm would stain just as dark as the membranes. But on fat, where there isn't much there except the membrane and the nucleus, it should work well.
> From: email@example.com on behalf of Göransson, Melker
> Sent: Monday, March 17, 2008 2:36 AM
> To: firstname.lastname@example.org
> Subject: [Histonet] Strong dye for adipose tissue cell membranes
> Hi all,
> I need a strong dye to stain cell membranes in 5µm sections of paraffin embedded adipose tissue. Prolonged Htx-E is not enough in order to perform computer based size measurements using the software i got. I am quite unexperienced in the histology field, is there a strong stain suitable for staining of cell membranes ( nuclei may stain as well ) ??
> Thank You/
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