Always a pleasure to help out a fellow Wisconsinite.
We use Hep. B surface from Thermo Scientific (manufactured by NeoMarkers
for Lab Vision Corp.), cat. # MS-314-S0 (0.1 ml), or -S1 (0.5 ml), or -S
(1.0 ml) for the supernatant concentrate. We use it at a 1:15 dilution
on our Ventana stainers with no pretreatment. This antibody is an ASR.
Their number is: 1(800)828-1628.
Hope this helps,
Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792
[mailto:firstname.lastname@example.org] On Behalf Of Bliven,
Sent: Monday, March 17, 2008 8:50 AM
Subject: [Histonet] Antibody for HBsAg
I'm looking for a good Hepatitis B Surface Antigen antibody. Must be
ASR. I know recently some companies changed from IVD to ASR or RUO. In
our clinical setting it must be at least ASR. Also, I understand that
it matters whether we use a polyclonal or monoclonal antibody. Should I
assume that the antibody must be polyclonal for this virus as some
others viruses require it, so it's not too specific and may miss
So, the questions are:
which antibody for HBsAg that's ASR (no RUO labeled)
and polyclonal or monoclonal?
Antibody consistency from the company is important as we use
1000 N. Oak Ave.
Marshfield, WI 54449
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