RE: [Histonet] Antibody for HBsAg

From:"Sebree Linda A."

Hi Laura,

Always a pleasure to help out a fellow Wisconsinite.  

We use Hep. B surface from Thermo Scientific (manufactured by NeoMarkers
for Lab Vision Corp.), cat. # MS-314-S0 (0.1 ml), or -S1 (0.5 ml), or -S
(1.0 ml) for the supernatant concentrate.  We use it at a 1:15 dilution
on our Ventana stainers with no pretreatment.  This antibody is an ASR.
Their number is:  1(800)828-1628.

Hope this helps,


Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

-----Original Message-----
[] On Behalf Of Bliven,
Sent: Monday, March 17, 2008 8:50 AM
Subject: [Histonet] Antibody for HBsAg

I'm looking for a good Hepatitis B Surface Antigen antibody.  Must be
ASR.  I know recently some companies changed from IVD to ASR or RUO.  In
our clinical setting it must be at least ASR.  Also, I understand that
it matters whether we use a polyclonal or monoclonal antibody.  Should I
assume that the antibody must be polyclonal for this virus as some
others viruses require it, so it's not too specific and may miss
positive cases?

So, the questions are:
which antibody for HBsAg that's ASR (no RUO labeled)
and polyclonal or monoclonal? 
Antibody consistency from the company is important as we use
concentrated antibodies. 


Laura Bliven
Histology Lab/IHC
Marshfield Laboratories
1000 N. Oak Ave.
Marshfield, WI 54449

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