Sudan black B shouldn't bleed. After staining,
rinse in 70% alcohol until non-lipid structures
are destained, wash in water, and mount in an
aqueous medium. The slides are good for months -
probably longer.
-- 
-------------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
_______________________________
Sharon E Willman wrote:
> 
> Hi,
> I am working on the Sudan Black stain.  Do the slides need to be read
> immediately due to bleeding of the stain after approximately 24 hours?
> 
> Thanks for any information you can give me about the stain.
> Sharon Willman
> 
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