Re: [Histonet] Somewhat rhetorical question regarding antibody incubation time

From:Gayle Callis


Probably depends on what kind of fixation used too? PFA?  NBF?

It is always fun to try to beat a recommended time frame with a dilution 
panel starting at 10 ug/ml and/or 4 hrs instead of overnight.

So many options to play with -

At 05:51 AM 3/17/2006, you wrote:
>If you have an antibody that the manufacturer recommends overnight
>incubation at 4C at 1:1200 dilution -
>is there anything to be gained from increasing the concentration (1:600)
>and incubating for one hour RT instead?

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717

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