RE: [Histonet] Gram's iodine mystery solved

From:"Smith, Allen"

   Shockingly, labels are not always correct. I have a jar labelled
"indigocarmine" whose contents is insoluble in water.
   I once (20 years ago) opened a brand new bottle labelled "acetic
anhydride" and caught a whiff of isoamyl acetate.  The contents also boiled
at the boiling point of isoamyl acetate (4 C higher than acetic anhydride),
and it did not dissolve in hot water.
   I have also borrowed a bottle of bromine from a colleague (25 years ago
at another school) and found that it performed brominations fairly well, but
required an elevated temperature to do them.  I never did find out what the
contaminant was or how it got there.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
[] On Behalf Of John Kiernan
Sent: Thursday, March 16, 2006 11:43 PM
To: meint002
Subject: Re: [Histonet] Gram's iodine mystery solved

Is this the end of the matter? Who swapped your 
iodine for iron filings? Do you have an enemy 
doing horrid things with the chemicals on your 
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
meint002 wrote:
> Dear Histos,
> Thanks to all for your advice about making Gram's Iodine soln.
> There was something wrong about the reagent iodine I was using.  I
> the proper iodine from another histo lab (thanks Luann) and now all is
> fine.  Dissolving it in the Potassium iodide worked like a charm.
> Joyce Meints
> Histologist
> University of Minnesota
> Paul and Sheila Wellstone Muscular Dystrophy Center
> MMC 206
> 420 Delaware St. SE
> Minneapolis, MN 55455-0392
> e-mail:
> lab phone: 612-626-4703
> _______________________________________________
> Histonet mailing list

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