Re: [Histonet] Normal solution
Good explanation Rene!
But I would just caution Dolors to ALWAYS add acid to water never
water to acid (too much heat generated-could erupt all over you). Add
the 82.9 ml of conc. HCl to say 800 ml of water, then quench to 1 L.
Date sent: Fri, 10 Mar 2006 08:36:38 -0800 (PST)
From: Rene J Buesa
To: firstname.lastname@example.org, email@example.com
Copies to: Subject: Re: [Histonet] Normal solution
> No, you cannot. The thing is that in order to have the needed MASS
> from a liquid chemical you have to introduce in the calculation 2
> other variables:
> 1- density of the chemical (= g/mL), and
> 2- concentration (% of the chemical in liquid form).
> For example: lets suppose you want to prepare a 1 Normal solution of
> HCl (hydrochloric acid). In this case: the molecular weight (MW) =
> 36.5 the density = 1.19 g/mL (find that in the label) and purity (or
> concentration) = 37% (also found in the label). So in order to have
> a 1N solution of HCl you will need:
> 36.5 g DIVIDED by (1.19 g/mL x 0.37) = 82.9 mL of H Cl + distilled
> water UP TO 1,000 mL Hope I have been apble to explain it! René J.
> Dolors Fuster wrote:
> *Hi histonetters!
> I was looking in archives how to made a Normal solution and found the
> following explanation:*
> ok....here we go. a 1N solution equals 1 gram-equivalent weight of
> compound in a liter of water. the gram-equivalent weight is determined
> by dividing the molecular weight by the number of hydrogen ions (or
> -OH groups) per formula. so, in the case of sodium hydroxide
> (NaOH....a single hydroxyl group) the gram-equivalent weight is 40g
> (ok....i'm rounding a bit for simplicity). so a 1N solution would be
> 40g/L.....therefore, a 2N solution would be 80g/L and a 5N solution
> would be 200g/L.
> carrie kyle-byrne
> *It's a really good explanation for me but I still have a question.
> Have I to follow the same procedure with liquids? What have I to do if
> I need a Normal solution of hydrocloric acid, nitric acid,
> iso-propylic alcohol or any other liquid compound? Have I to use the
> same amount of ml than gr used with solids applying the same formula?
> Thanks in advance
> Dolors Fuster
> Tècnic Especialista en Anatomia Patologica i Citologia
> Facultat de Medicina
> Dep. d'Anatomia i Embriologia Humana
> Universitat de Barcelona*
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