I'm soliciting advice about the best ways to examine fat tissue histologically.
We are preparing fat tissue for autografting (reinjection through a needle) and
want to look at damage to the fat tissue caused by extrusion through a needle.
Subcutaneous fat is surgically excised, scraped to remove most of the fibrous
component, stuffed into a syringe and then extruded out through a fine needle
under considerable pressure. I would appreciate any suggestions about the best
ways to compare normal fat tissue with the extruded fat 'goop'. In particular we
would like to be able to quantify the number of viable cells. Thanks, Jim
Kobler, Ph.D., Dept. of Surgery, Harvard Medical School
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