We use the rabbit monoclonal from Labvision:

Citrate Buffer HIER 10' in a microwave @ 95 degrees C, cool 10'
Nonserum block - 10'
Ki67 1:200 - 1 hour @ RT or overnight @ 4 degrees C
Immunovision rabbit polymer HRP - 30 minutes @ RT
DAB+

Others use Proteinase K enzyme digestion with this antibody with good
results.

Best wishes,

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133

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