[Histonet] Revealing lymph nodes


Terry Marshall notes having had no success with revealing lymph nodes by post-fixation in Davidson's fixative.
Neither have I. The clearing fixative must be used as the initial fixative. 
An old method is to pass the formalin-fixed tissue through alcohols into xylene (or cedar oil). I've never used this method, and the one time I've seen it used it didn't work. 
Once in a while there are no more than three lymph nodes in the axillary contents from a breast cancer. Your surgeon should live with it.
Bob Richmond
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