Dear Histonetters,
I have perfused rat brain with 4% paraformaldehyde, placed in fix O/N 
and then added a 10% and 30% sucrose solution. What is the next step 
toward long-term storage of this tissue if I plan on using a cryostat to 
section? How would you recommend storing this tissue and what is the 
next step after sucrose. I will not be sectioning for a few weeks. Also, 
how do I store the slides after sectioning? Do I let them dry for a few 
hours like I do with fresh tissue?

Thanks for your help,
Kelly McQueeney

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet