[Histonet] RE: Storage after perfusion of brain tissue, what is the best met hod?

From:"Vanko, Amy"

Hi Kelly,

You should store them in the minus 20 freezer.  I don't think that sucrose
can cryoprotect much colder than that.  

As far as saving sections, I have only done free-floating immuno on perfused
sections so we saved our sections in a cryoprotectant solution in the minus
20 freezer.  This was for stereology, so we actually saved one section/well
in 96 well plates.  

Amy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kelly D
Mcqueeney
Sent: Thursday, March 31, 2005 10:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Storage after perfusion of brain tissue, what is the
best method?


Dear Histonetters,
I have perfused rat brain with 4% paraformaldehyde, placed in fix O/N 
and then added a 10% and 30% sucrose solution. What is the next step 
toward long-term storage of this tissue if I plan on using a cryostat to 
section? How would you recommend storing this tissue and what is the 
next step after sucrose. I will not be sectioning for a few weeks. Also, 
how do I store the slides after sectioning? Do I let them dry for a few 
hours like I do with fresh tissue?

Thanks for your help,
Kelly McQueeney

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