RE: [Histonet] disinfection of cryostat

From:Tony Henwood


100% ethanol is a poor disinfectant. 70% is better. The 30% water content is
need for the ethanol to be reactive with the various pathogens that ethanol
is effective against. Unfortunately the water content may result is ice
formation but a good wipe with a dry rag could be used.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: Michael Hetzer [] 
Sent: Wednesday, 2 March 2005 8:23 AM
Subject: [Histonet] disinfection of cryostat

Use 100% ETOH to disinfect your cryostat without turning it off. We use
the last of the dehydrating ETOH from the previous day's staining
setup.Wear your PPE and use 4x4 gauze  that is soaked with the ETOH to
the point of dripping a little bit. Dispose of your blade first or if
your using a knife first disifect both sides of the kife using only
upward wipes and then set the knife in a safe out of the way place
inside the cryostat preferrably a spot that has already been cleaned.
Remove any waste materials with the wet gauze and then wipe the inner
surfaces.Wipe out any excess ETOH with dry 4x4 gauze. It will take you
five minutes at the most.

Mike Hetzer
Dermatologic Procedures
Madison, WI

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