[Histonet] decalcifying embedded tissues
I am studying gonad morphology of frogs. I usually decalcify then embed
the whole animal (because they are tiny). However, I recently forgot to
decalcify (several samples). I have ordered and soaked many of my samples
in RDO, but I am still having a hard time sectioning them. They are either
scratched along the surface or "falling out" or the paraffin...
Are their any suggestions on how I can de-calcyfy these specimens? Should
I take them out of the block and soak them then re-embed?
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