I fancy it involved removing the paraffin and taking back to thin cedar wood
oil (which is a 'clearing agent') and soaking for a few hours. Then you
remove the cedar wood oil with three changes of molten paraffin. We also
used 'Mollyfex' but I can't remember who sold it; the problem over taking
tissue right back to water is that you have to dehydrate and clear again,
all that hardens tissue. If the tissues are irretrievable then give it a
try; but only after you have tried to get a diagnostic section. If you
really want to sort it out then read 'Histopathologic Technic and Practical
Histochemistry' RD Lillie and Harold M. FullmerMcGraw-Hill Inc 1975.
Histology is becoming a bit like computers I think; I grew up with MSDOS and
DRDOS and can still 'shell out' from Windows XP. Automatic tissue processors
mean we are losing the 'art' in Histology; the grumblings of an old man.

 

I suppose buying Lillie is like buying a book on MSDOS too; you never know
when you need to go back to basics.    

 

Kemlo Rogerson

Cellular Pathology Manager

East Lancashire Hospitals NHS Trust

DD. 01254-294162

Mobile 0774-9754194

 

-----Original Message-----
From: Jennifer Sipes [mailto:jengirl1014@yahoo.com] 
Sent: 16 March 2005 12:34
To: Kemlo Rogerson
Subject: RE: [Histonet] processing[Scanned]

 

I'd greatly appreciate that.  What is this Lillie book?  I don't think I
have that one.  Would it be a good idea to purchase it?  Thanks again for
your help.

Kemlo Rogerson  wrote: 

 

Jennifer K. Sipes, RALAT

Sr. Laboratory Technician

Johns Hopkins University

Ross 929

720 Rutland Avenue

Baltimore, MD  21205

phone:  410-614-0131

cell:     443-413-0853

e-mail:  jengirl1014@yahoo.com  

 

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