RE: [Histonet] Isopropanol tissue processing
I think the company that sells the Bond Immunostainer (Vision
Biosystems) has developed a process that makes replacing Xylene with
Isopropyl Alcohol possible. It has something to do with the heat I
think. I was talking with their rep James about this. I admit I still
have a lot of questions about the process, but apparently it is
Ross M Stapf
Baylor University Medical Center
3500 Gaston Ave.
Dallas, TX 75246
[mailto:firstname.lastname@example.org] On Behalf Of Gayle
Sent: Thursday, March 18, 2004 10:01 AM
To: Histology; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Isopropanol tissue processing
We used isopropanol was used in place of ethyl alcohol as a dehydrant,
but never replaced the xylene step just before paraffin with it. I
don't think isopropyl alcohol is miscible with paraffin. My Merck index
says isopropyl is miscible with water, alcohol, ether and chloroform
(carcinogenic) but nothing said about paraffin.
After isopropyl gradient we cleared with xylene. You will still need a
clearant, ie. xylene or one of the xylene substitutes to remove alcohol
before infiltration with paraffin. Maybe a better solution is to find a
better clearant substitute before paraffin.
At 11:42 AM 3/18/2004 +1100, you wrote:
>Does anyone use Isopropanol in routine overnight parraffin processing
replacing xylol?? If you are willing could you please supply any
protocols you may use.
>Thanks for your help.
>Sydney, NSW,Australia _______________________________________________
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Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
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