Re: [Histonet] Isopropanol tissue processing

From:Gayle Callis

We used isopropanol was used in place of ethyl alcohol as a dehydrant,  but
never replaced the xylene step just before paraffin with it.  I don't think
isopropyl alcohol is miscible with paraffin.  My Merck index says isopropyl
 is miscible with water, alcohol, ether and chloroform (carcinogenic) but
nothing said about paraffin.  

After isopropyl gradient we cleared with xylene.  You will still need a
clearant, ie. xylene or one of the xylene substitutes to remove alcohol
before infiltration with paraffin.  Maybe a better solution is to find a
better clearant substitute before paraffin.


At 11:42 AM 3/18/2004 +1100, you wrote:
>Hi There
>
>Does anyone use Isopropanol in routine overnight parraffin processing
replacing xylol?? If you are willing could you please supply any protocols
you may use.
>Thanks for your help.
>
>Frances
>SDS Pathology
>Sydney, NSW,Australia
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>
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology 
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)



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