(Fwd) Re: [Histonet] EDTA pH 8.0 recipe
Hi Tammy,
I maintain my own "personal" Histonet Archives, and I found the
following post from 1999. Good luck.
Greg
Date sent: Mon, 27 Dec 1999 11:15:29 -0600
From: "Lott, Robert"
Subject: EDTA-NaOH, pH 8.0 retrieval fluid
Forwarded to: DOBBIN@acad1.cs.upei.ca
To: "'histonet@pathology.swmed.edu'"
Stock Solution:
1.86 gm. EDTA (same as used for decalcification)
5.0 liters DI water
To the above solution, add 2 to 2.5 ml. 2N NaOH.
Adjust solution to pH 8.0 with 1N NaOH or 1N HCl solution.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
From: "Barnhart, Tammy"
To: "Histonet (E-mail)"
Date sent: Wed, 24 Mar 2004 07:57:44 -0600
Subject: [Histonet] EDTA pH 8.0 recipe
> Does anyone have a recipe to make EDTA buffer at a pH of 8.0. I would like
> to make our own in an effort to save some dollars. I have checked the
> archives and only have come up with one rather difficult procedure. Thanks
> in advance....
>
> Tammy Barnhart, BS, HTL(ASCP)
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> Bismarck, ND
> tbarnhart@primecare.org
>
>
>
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