Tammy and Greg
EDTA powder is only slightly soluble in water. I prefer to start with
disodium EDTA (that is much more soluble)and then adjust the pH to 8
with NaOH.
Can also use this in saline.
The 1.86 gms in 5 liters is a very weak solution for demineralization.
Normally we use 5% EDTA adjusted to pH 7.3.
If cost is a the determining factor then EDTA can be recovered from a
used demineralization solution - however very few do this because of the
time involved.
Barry


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Greg
Dobbin
Sent: Wednesday, March 24, 2004 5:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: (Fwd) Re: [Histonet] EDTA pH 8.0 recipe


Hi Tammy,
I maintain my own "personal" Histonet Archives, and I found the 
following post from 1999. Good luck.
Greg

Date sent:      	Mon, 27 Dec 1999 11:15:29 -0600
From:           	"Lott, Robert" 
Subject:        	EDTA-NaOH, pH 8.0 retrieval fluid
Forwarded to:   	DOBBIN@acad1.cs.upei.ca
To:             	"'histonet@pathology.swmed.edu'" 



Stock Solution:
	1.86 gm. EDTA (same as used for decalcification)
	5.0   liters DI water

To the above solution, add 2 to 2.5 ml. 2N NaOH.
Adjust solution to pH 8.0 with 1N NaOH or 1N HCl solution.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From:           	"Barnhart, Tammy" 
To:             	"Histonet (E-mail)"

Date sent:      	Wed, 24 Mar 2004 07:57:44 -0600
Subject:        	[Histonet] EDTA pH 8.0 recipe

> Does anyone have a recipe to make EDTA buffer at a pH of 8.0.  I would

> like to make our own in an effort to save some dollars.  I have 
> checked the archives and only have come up with one rather difficult 
> procedure. Thanks in advance....
> 
> Tammy Barnhart, BS, HTL(ASCP)
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> Bismarck, ND
> tbarnhart@primecare.org
> 
> 
> 
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