fixing and decalcifying

From:Millicent Pope

Hi!  I have a question I'm hoping someone out there can answer.  I have
dog teeth (still in the bone) to fix and decalcify for paraffin
sectioning and H&E staining.  I've had problems in the past with the
pulp in these teeth being too soft.  I would like to try to cut the
teeth in half and fix them longer (in Davidson's fixative) before
decalcifying them the rest of the way and processing them.  What would
be a good way to go about this?  

Thanks-

Milli Pope
VAPH Histology



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