Calbiochem. Rabbit anti-human factor XIII subunit
a.
Dilute 1:400 in PBS with 1% bovine serum
albumin.
For FFPE tissues trypsin (I use Sigma tablets)
digestion for 15-30 minutes is requires. HIER is too much and causes
background.
I just went to Calbiochem website and can't find
the Factor XIIIa. Biogenex and Biocare Medical have one, just follow their
dilution recommendations and my antigen retrieval recommendations and you
should be alright. Feel free to email me if you have trouble, this is part
of my research interest.
Jeff Silverman HT, HTL, QIHC
(ASCP)
Southside Hospital
Bay Shore NY USA