Re: Liquid nitrogen problem
Still use iso pentane but cool it in a bath containing dry ice and
IMS. You will get the temperature down to about -70. Your freezing should
be fine because the critical factor is to get the temperature below -20 as
quickly as possible to avoid ice crystal formation. I have use this method
many times and never had a failure. If you are worried try out a test
sample first before getting on with your precious biopsies.
Asthma Biology Department.
GlaxoSmithKline Medicines Research Centre,
tel. +44 (0)1438 764117
fax. +44 (0)1438 764782
mobile +44 07753609835
Subject: Liquid nitrogen problem
I am new to this work and if somebody could help me with this
problem I would be very grateful.
We are going to obtain skeletal muscle samples from surgery
which we want to use for immunohistochemistry and in situ
hybridization. We wanted to mount the muscle sample on cork in
OCT and freeze it in isopentane/ liquid nitrogen there at the
hospital. However there is no facility for getting liquid nitrogen at
the hospital and transporting it there would be difficult. My question
is would it be possible to freeze the muscle sample on dry ice,
transport it back to the lab and then freeze in isopentane/liquid
nitrogen? Could this sample still be used for immunohistochemistry
and in situ hybridization?
Thanks to anyone that can help!
University College Dublin,
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