Good Paraffin for TMA's
Hi Histonetters,
I have run into a problem with something to do with
the embedding process. I have bubbles in the blocks after they come off the
embedding center. I'm not sure if it's air in the line of the new
embedding center (Sukura) or with the paraffin (Shandon). Has anyone
experienced any problems with the new embedding center? Could that be what
is making these bubbles? Is it the paraffin, (I don't think so), has
anyone seen these bubbles form in paraffin as the block cool or even the next
day? Lastly any suggestions if I have to change paraffins as to which to try,
needs to be hard to core yet we have to be able to produce good sections at 4
microns for H&E's. I don't process the tissue on site, so It's hard to
trouble shoot but I have never seen these bubbles and I have been around for a
long time.
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