I'm not sure if the over night (24hr) drying of sections is good for staining. I've done that before because I had the same problem: tissue falling off the slides during rehydration. But all what I got is excessive background, no matter how low I went on the dilutions. However, Mari's suggestion on "baking" the slides in an oven (I do it at 55-60C) helps a lot. But like I mentioned, nothing beats superfrost, poly-lysine (positively charged) coated slides. Hope this is helpful.

Nidal E Muvarak
Associate Research Specialist
Department of Biomedical Engineering
University of Wisconsin-Madison
1550 Engineering Dr.; Rm. 2158
Madison, WI 53706-1609
Lab: (608) 265-8921; Office: (608) 265-4205; 
Home: (608) 256-7934; Cell: (608) 332-6068

----- Original Message -----
From: mari.ann.mailhiot@leica-microsystems.com
Date: Thursday, March 6, 2003 9:22 am
Subject: Re: 40 um paraffin sections coming loose from the slides

> 
> Susanne
> 
> It is a good idea with very thick sections of brain to let them=20
> air dry for
> 24 hr. Then place the slides in an over to melt paraffin. Keep 
> using the
> slides you are using. I found them to be the best.
> 
> Regards
> 
> Mari Ann Mailhiot BA HT ASCP
> Applications Specialist
> Leica Technical Assistance Center
> 800 248 0123
> 
> 
> 
> 
> Susanne Sørensen  on 03/05/2003 03:53:23 PM
> 
> To:    histonet 
> cc:
> 
> Subject:    40 um paraffin sections coming loose from the slides
> 
> 
> Hi Everyone
> 
> I'm cutting 40 um paraffin sections, human brain, so the sections=20
> is very
> big. The problem is, that some sections (to much) is coming loose=20
> from the
> slides when I rehydrate. I coated the slides with 4,5% gelatine=20
> and 4,0%
> potassium chromic sulphate.  I have tried silane slides but the
> gelatine/ChromeAlun seems to be the best for now.
> 
> If anyone has a suggestion to overcome the problem with loose 
> section, I
> would like to here from you.
> 
> Thanks
> 
> Sus Sørensen
> Research Lab Stereology & Neuroscience
> Denmark
> susr@city.dk
> 
> 
> 
> 
> 
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