RE: Dark blue mucin in an H&E

From:"Smith, Allen"

I use Ehrlich's hematoxylin.  It does not stain goblet cell mucin when it is
When it is a few weeks old, it starts to stain goblet cell mucin.  I have
always assumed [n.b., assumed] that this was due to a slow rise in the pH of
the staining solution.

Allen A. Smith, Ph.D.
Barry University
School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161-6695 

-----Original Message-----
From: Bill []
Sent: Wednesday, March 12, 2003 9:00 AM
Subject: Dark blue mucin in an H&E

My histotech is out for a week or 2 and my substitute is producing 
colon biopsies in which the mucin stains deep blue with H&E.

I have seen this many times over the years, but have never figured 
out its cause. The substitute is not a good trouble shooter.

Does anyone know what causes this abnormal staining in goblet cells?


Wm F Blank MD
Heartland Laboratory, Inc
Chaffee, MO 63740

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