RE: Dark blue mucin in an H&E
I use Ehrlich's hematoxylin. It does not stain goblet cell mucin when it is
When it is a few weeks old, it starts to stain goblet cell mucin. I have
always assumed [n.b., assumed] that this was due to a slow rise in the pH of
the staining solution.
Allen A. Smith, Ph.D.
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161-6695
From: Bill [mailto:firstname.lastname@example.org]
Sent: Wednesday, March 12, 2003 9:00 AM
Subject: Dark blue mucin in an H&E
My histotech is out for a week or 2 and my substitute is producing
colon biopsies in which the mucin stains deep blue with H&E.
I have seen this many times over the years, but have never figured
out its cause. The substitute is not a good trouble shooter.
Does anyone know what causes this abnormal staining in goblet cells?
Wm F Blank MD
Heartland Laboratory, Inc
Chaffee, MO 63740
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