My lab's been using AEC as the color substrate of choice for several years.  The color was stable for at least 6 months until very recently.  I'm seeing that some staining lasts 6 months while others only last a onth or so.  I cover slip using glycerol gelatin.  Any thoughts as to why the shift in stability?  I've tried using different batches of AEC with no change.

Those of you using DAB...can I use a water soluble mount with DAB?  I'm not a histo-buff...this is just a side part of my job so please pardon my obvious lack of basic knowledge.

Thanks!
Sylvia Poulos
Animal Physiology Research Unit
Athens, GA