My lab's been using AEC as the color substrate of choice for several years. The color was stable for at least 6 months until very recently. I'm seeing that some staining lasts 6 months while others only last a onth or so. I cover slip using glycerol gelatin. Any thoughts as to why the shift in stability? I've tried using different batches of AEC with no change.
Those of you using DAB...can I use a water soluble mount with DAB? I'm not a histo-buff...this is just a side part of my job so please pardon my obvious lack of basic knowledge.
Animal Physiology Research Unit
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