Thanks to all you responded on the quality of perfusion question.  

Several suggested a panel of judges, a couple said staining for Vimentin
would be an indication.  Like HRP in animals, it doesn't work at all if
the tissue is not fixed, but gets weaker and weaker with overfixtion.
Hard to put two slides side by side and say, this proves that one is
better fixed. In fact, it seems that even the powerful Histonet does not
have an answer to a question as fundamental to our profession as how to
prove one slide has tissue that is better perfused and fixed than
another.  It is after all an art.

Cordially,

Charles W.  Scouten, Ph.D.
myNeuroLab.com
5918 Evergreen Blvd.
St. Louis, MO 63134
Ph: 314 522 0300  
FAX  314 522 0377
cwscouten@myneurolab.com
www.myneurolab.com