brain infarct responses
Fellow Histonetters, I would like to thank those
that responded to my question in regards to my posting for brain
infarct special stains. I did forget to mention the brain would be
embedded in paraffin.
I thought I would share with you the responses I
received:
1) Depending on how long after injury, but if
more than 24 hours you should get good results with Thionin and Acid Fuchsin
staining.
2) I am assuming this is a blood clot so it
may be something like a general stain that will pick up the blood cells.
You could try a quick and dirty methylene blue or toluidine blue at .5 to 1% and
see what it shows or if you need a more indepth stain for the brain
architecture.
3) In my lab we use the HBFP stain, hematoxylin-basic
fuchsin-picric acid stain for myocardial infarcts from Sheehan and Hrapchak
histo tech book. I have tried it on splenic infarcts and it worked. Maybe
it could work for neural infarcts.
Thanks again :-)
Cathy
*********************************
GLP Compliant
Laboratory
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