Re: azure B


i use azure b all the time but not to stain melanin.  i use it to stain bone, cartilage and collagen.
it is a very simple stain
0.2% azure b
i use it at different ph's for different tissue components
for you i would use at ph 6-7, then it stains about everything
if you just wanted cartilage to stain use an acid ph about 1.2
ph 4 will stain cartilage and bone
ph 7 stains most components

make up the stain and ph it to what you want
stain the sections for 5 min., pour off the stain and save it (do not filter)
rinse the slides til clear in deionized h20
at this point i air dry and coverslip with permount without going thru alcohols and xylene, the solvents will remove the stain
we love this stain, it is so easy and versatile


"Wilhelms, Margaret B" wrote:

> Does anyone have a procedure for using azure B to stain melanin on paraffin
> embedded sections?  Has anyone heard of this?
> Thanks
> Meg
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