Re: Antibody for ELISA to use for ICC


i often use antibodies for IHC which are recommended only for ELISA.  you
usually have to use the antibody at a more concentrated protein concentration
for sections as compared to ELISA.  do some dilution titration studies to
determine what works for sections.  i always start out with a one hour
incubation time for the antibody for sections.

Jasmine Piep wrote:

> Hi everyone,
> I have big questions. I have a bad luck in finding a Mono. or Poly
> anti-mouse Albumin that can work on ICC. It oly can work for
> Western-blotting, ELISA, and/or Immunoprecipitation. Most are good for
> ELISA. Do you think if I pick the antibody from one vendor that's for ELISA
> and use it for ICC? I have to make the choice soon, because my boss is very
> impatient.
> Jasmine
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