Cheryl,
I am wondering if you could be getting some antibody carry over from the DAKO
probe or check the program carefully to see that the right vial for your neg. is
being accessed.
Patsy

"George, Cheryl" wrote:

> I'm using the patient tissue as a negative control, not applying the primary
> antibody.  We use the Dako autostainer and have not had problems with any
> other antibodies.  The first time this happened, the sample was a cell block
> of a pleural fluid, the second time a lung biopsy.
>
> > ----------
> > From:         Carrie Kyle-Byrne[SMTP:ckbyrne@exelixis.com]
> > Sent:         Tuesday, March 26, 2002 3:34 PM
> > To:   George, Cheryl
> > Cc:   'HistoNet@Pathology.swmed.edu'
> > Subject:      Re: Calretinin
> >
> > what are you using for your negative control and how are you doing your
> > staining (manual or automated...which machine)?  what type of tissue is
> > the
> > patient specimen?
> > carrie
> >
> > ----- Original Message -----
> > From: "George, Cheryl" 
> > To: 
> > Sent: Tuesday, March 26, 2002 10:57 AM
> > Subject: Calretinin
> >
> >
> > > Hi out there,
> > >    We do calretinin on human FFPE tissue and over the last couple of
> > weeks
> > > have had two cases where the patient negative control tissue stains just
> > as
> > > brightly and specifically as the positive.  Other markers on the same
> > > patient tissue don't react this way and I was wondering if anyone has an
> > > idea about what could cause this.
> > >
> > > Thanks in advance!!
> > >
> > > Cheryl
> > >
> > > Cheryl George, BS, HT (ASCP)
> > > Anatomic Pathology Supervisor
> > > Elliot Hospital Laboratory
> > > One Elliot Way
> > > Manchester, NH 03103
> > > (603) 663-2686
> > >
> > >
> > >
> > >
> > >
> >