RE: processing brain sections


Hi All,
Andi Grantham and I will be presenting a lecture at NSH in Long Beach this
year called; Let Alcohol Clear Your Brain.  It deals with brain processing
and we discuss many of the problems routinely encountered with CNS tissues.
If you can be there please attend, if not you may contact either of us by
e-mail at: for Andi or for Pam Marcum.  We will be happy to share any of
the protocols we have worked out or just listen to the horror stories and
attempt to help or direct you to help.  Pam Marcum

> -----Original Message-----
> From: Anacko, Nancy L. []
> Sent: Wednesday, March 20, 2002 8:38 AM
> To: 'Server, Histonet'
> Subject: processing brain sections
> Hi,
> Can someone help me ?? How should my processor be set up to
> process brain ??
> Currently
> I use 2 cont. 10% neutral buffered formalin, 1 cont. 70% alc., 1 cont. 80%
> alc., 2 cont. 95% alc., 2 cont.100% alc., 2 cont. xylene and of
> course 2 wax
> chambers. I couldn't tell you the times in each because the screen on my
> shandon is burnt out, I'm new to the dept. and can't find our procedure.
> Soooo if anyone could send me their would be appreciated.
> Maybe I could get my processor fixed or a new one (hi,diane if you're
> reading this :))
> thanks :)

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