Hi,

Does anyone have any protocols for B-Gal staining of
mouse growth plate/leg bone in frozen sections? What
thickness should I cut the bone to get the best
morphological results? I usually cut 20 micron
sections for tissue, but I am assuming that is much
too thick for bone! Also, what age of mouse is best? I
have cut frozen bone samples from pups up to 7 days
old, but am wondering if it is possible to use bones
from adult mice.

Thanks in advance for the input!

Regards,

Elizabeth Lummus
Biochemistry
U.T. Southwestern Medical Center at Dallas
USA
214-648-5063 

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